| Gene | Ttll6 |
| Chromosome | 11 |
| Deletion Start | 96,158,956 |
| Deletion End | 96,138,860 |
| Deletion Size | 20,097 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 96,138,969 | 96,138,988 | GGCAGAGGTGGAGATGAAAC |
| TUP | 96,138,989 | 96,139,011 | TGAGCCGGTCTCGTGTCTTGGAT |
| TUR | 96,139,035 | 96,139,053 | GGGTGAGGGACATTGTGAC |
| TDF | 96,158,510 | 96,158,529 | TCCCAGCTCTCGTTCCTATG |
| TDP | 96,158,533 | 96,158,559 | CCCATCATCTGTGCCAATCTTGGGTGA |
| TDR | 96,158,561 | 96,158,584 | TCTCTGCTCCACTCAGTTTCTTTG |
PCR Primers
| Primer | Sequence |
| SU | GGACCTTATCGGTAGCTCCTC |
| SD | TTCCTCTGTCCTGTCATCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17697A-A1 | VGB6 | C57BL/6NTac | |
| 17697A-A4 | VGB6 | C57BL/6NTac | |
| 17697A-A5 | VGB6 | C57BL/6NTac | |
| 17697A-B12 | VGB6 | C57BL/6NTac | |
| 17697A-G2 | VGB6 | C57BL/6NTac | |
| 17697A-F4 | VGB6 | C57BL/6NTac | |
