| Gene | Tube1 |
| Chromosome | 10 |
| Deletion Start | 39,149,618 |
| Deletion End | 39,134,086 |
| Deletion Size | 15,533 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 39,134,372 | 39,134,392 | GACCTGGGACTTGCTGTGTAG |
| TUP | 39,134,400 | 39,134,426 | TCTGGAATTTGCAGCGATCCATCTGTC |
| TUR | 39,134,437 | 39,134,458 | GGGCATGATAGTGGTAGTCCTG |
| TDF | 39,149,535 | 39,149,557 | CGCTCATACAGGAGTACAGTGAC |
| TDP | 39,149,558 | 39,149,580 | CTCGATGCCACAAAGAGCCTGCC |
| TDR | 39,149,586 | 39,149,607 | ACACTCAACCTGGGCACATCTG |
PCR Primers
| Primer | Sequence |
| SU | CAGCTAGGATTTACCTGCCG |
| SD | GACGACTGCTCCTTCCCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17698A-A3 | VGB6 | C57BL/6NTac | |
| 17698A-C7 | VGB6 | C57BL/6NTac | |
| 17698A-D6 | VGB6 | C57BL/6NTac | |
| 17698A-G7 | VGB6 | C57BL/6NTac | |
| 17698A-G2 | VGB6 | C57BL/6NTac | |
