| Gene | Ube2b |
| Chromosome | 11 |
| Deletion Start | 52,000,333 |
| Deletion End | 51,986,680 |
| Deletion Size | 13,654 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 51,999,494 | 51,999,515 | ACCTCTCCCAGTTGTTTACCTC |
| TUP | 51,999,466 | 51,999,493 | TCCTTGGAAACGTACAAGTGCAGAAACA |
| TUR | 51,999,427 | 51,999,445 | AGCGGGTCTGCCCAGAAAG |
| TDF | 51,987,408 | 51,987,429 | TTCCCACCTCAGAACTTTACTC |
| TDP | 51,987,378 | 51,987,405 | TTATTCCCTCCCTGCTAAGCGTATGTGA |
| TDR | 51,987,346 | 51,987,367 | CCGCTAGGCCCAAACCTTATTC |
PCR Primers
| Primer | Sequence |
| SU | AGCGGATCCTCCGTAGTTG |
| SD | AGACTTCGCAGTCCCTCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17699A-A11 | VGB6 | C57BL/6NTac | |
| 17699A-B5 | VGB6 | C57BL/6NTac | |
| 17699A-F9 | VGB6 | C57BL/6NTac | |
| 17699A-F4 | VGB6 | C57BL/6NTac | |
| 17699A-B12 | VGB6 | C57BL/6NTac | |
