| Gene | Ube2d1 |
| Chromosome | 10 |
| Deletion Start | 71,285,121 |
| Deletion End | 71,255,779 |
| Deletion Size | 29,343 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 71,284,510 | 71,284,533 | GCTGTACTGAGAACATAGTTGCTG |
| TUP | 71,284,483 | 71,284,507 | ACACAGCAAGATGAGCTCCTGGCTG |
| TUR | 71,284,458 | 71,284,481 | GCTTAGCTTACCTAGCCATGAATC |
| TDF | 71,256,275 | 71,256,295 | CCACCCATGCTGACCTTACTG |
| TDP | 71,256,250 | 71,256,274 | TCAAGCCACTGCATCCTCCTAGTGC |
| TDR | 71,256,216 | 71,256,240 | GGGAGGGTTATCTCCAAGATTCAAG |
PCR Primers
| Primer | Sequence |
| SU | CAGCCTGGTCGCAGCTGTC |
| SD | GTGACGACATCTTGGACGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17700A-A6 | VGB6 | C57BL/6NTac | |
| 17700A-B2 | VGB6 | C57BL/6NTac | |
| 17700A-C4 | VGB6 | C57BL/6NTac | |
| 17700A-H12 | VGB6 | C57BL/6NTac | |
| 17700A-C1 | VGB6 | C57BL/6NTac | |
