| Gene | Wdr12 |
| Chromosome | 1 |
| Deletion Start | 60,097,544 |
| Deletion End | 60,078,156 |
| Deletion Size | 19,389 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 60,097,497 | 60,097,521 | CAGCGAGAACAAGAAGTAAGTAAGG |
| TUP | 60,097,471 | 60,097,494 | TCCAGCTTCTGTCCCTGTCTTCCA |
| TUR | 60,097,428 | 60,097,451 | AGCAAGGATCAAATCGAAGGAGTC |
| TDF | 60,078,407 | 60,078,430 | CACTAGGGAAGCAGAGGATGACAG |
| TDP | 60,078,383 | 60,078,405 | TCTCAGAGCATGAAGCCAGCCCA |
| TDR | 60,078,351 | 60,078,373 | GCCTTCATTGTTCTGGAACTCAC |
PCR Primers
| Primer | Sequence |
| SU | TGAGCGCTGAGTCTAAAGGG |
| SD | GACAATGATATCAGAGGATTATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17706A-A11 | VGB6 | C57BL/6NTac | |
| 17706A-C6 | VGB6 | C57BL/6NTac | |
| 17706A-E3 | VGB6 | C57BL/6NTac | |
| 17706A-F9 | VGB6 | C57BL/6NTac | |
| 17706A-F3 | VGB6 | C57BL/6NTac | |
| 17706A-E4 | VGB6 | C57BL/6NTac | |
