| Gene | Wdr26 |
| Chromosome | 1 |
| Deletion Start | 181,209,290 |
| Deletion End | 181,177,757 |
| Deletion Size | 31,534 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 181,209,095 | 181,209,113 | TTGCAGGCAGAGAATGACC |
| TUP | 181,209,058 | 181,209,084 | AAAGCCTTTAGTGCATTCTCCTCACGC |
| TUR | 181,209,034 | 181,209,053 | TCAAGTGCGCCTCTTACCAC |
| TDF | 181,178,397 | 181,178,418 | CCAAATGGAGCTGCATGGTATG |
| TDP | 181,178,371 | 181,178,395 | TCGACTTGGAGCAGCTGAAGGAGTT |
| TDR | 181,178,326 | 181,178,347 | TGGTAGTATGAGGGAGGCAGAG |
PCR Primers
| Primer | Sequence |
| SU | GAGAAAGGAAAGGGTTTGGG |
| SD | TTCAATGGGTAAGCCTGAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17707A-A3 | VGB6 | C57BL/6NTac | |
| 17707A-A11 | VGB6 | C57BL/6NTac | |
| 17707A-D11 | VGB6 | C57BL/6NTac | |
| 17707A-H11 | VGB6 | C57BL/6NTac | |
| 17707A-F4 | VGB6 | C57BL/6NTac | |
