| Gene | Wdr35 |
| Chromosome | 12 |
| Deletion Start | 9,006,770 |
| Deletion End | 8,974,159 |
| Deletion Size | 32,612 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 8,974,294 | 8,974,315 | CCTATCTCCTCTCACAGGTCTC |
| TUP | 8,974,316 | 8,974,338 | TGTGCTCCGGTAGTCCTTGGCAG |
| TUR | 8,974,359 | 8,974,382 | GCATGACTGGAATGTGGAAGAATC |
| TDF | 9,006,334 | 9,006,351 | CTGGTGGTTGCAGGACTC |
| TDP | 9,006,358 | 9,006,384 | TTACCCTTCTGGTGTCACGTGCTTACC |
| TDR | 9,006,385 | 9,006,406 | AGCTATTAGAGCTGTGCTCAGG |
PCR Primers
| Primer | Sequence |
| SU | CATGGCAGATCGCTAAACTG |
| SD | GGAAAATACACCGGTTCATTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17708A-B11 | VGB6 | C57BL/6NTac | |
