| Gene | Wdr47 |
| Chromosome | 3 |
| Deletion Start | 108,644,685 |
| Deletion End | 108,609,889 |
| Deletion Size | 34,797 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 108,609,979 | 108,609,998 | CTGGCGCTGGAGAAGGAAAG |
| TUP | 108,609,999 | 108,610,020 | TGGAGTCATAAATGGCCTGTTT |
| TUR | 108,610,024 | 108,610,048 | CGTACCTCAGGAAGAGCATATCATC |
| TDF | 108,643,887 | 108,643,904 | TGAGGCAGGGCAGACTTG |
| TDP | 108,643,911 | 108,643,938 | TCGTGACACTGTAGGAAGTTGACGTGTT |
| TDR | 108,643,940 | 108,643,964 | TCTCTTTCCTGGGTTGACTATGTTC |
PCR Primers
| Primer | Sequence |
| SU | CTGTCACTGCTAGACTATTCTG |
| SD | GGATCGTGACAAGAGCAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17709A-B8 | VGB6 | C57BL/6NTac | |
| 17709A-G3 | VGB6 | C57BL/6NTac | |
| 17709A-B5 | VGB6 | C57BL/6NTac | |
| 17709A-D2 | VGB6 | C57BL/6NTac | |
| 17709A-D3 | VGB6 | C57BL/6NTac | |
| 17709A-F10 | VGB6 | C57BL/6NTac | |
