| Gene | Wrnip1 |
| Chromosome | 13 |
| Deletion Start | 32,822,213 |
| Deletion End | 32,802,241 |
| Deletion Size | 19,973 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,802,895 | 32,802,915 | AAGCCGCTGGCTGACAAGATG |
| TUP | 32,802,918 | 32,802,940 | TCCAGACACGCTGCAGGACTACA |
| TUR | 32,802,958 | 32,802,977 | AGCAGAGTCTCCTGCCCTAC |
| TDF | 32,822,117 | 32,822,138 | GGAGCTGAGAGGAGTTGACTTC |
| TDP | 32,822,139 | 32,822,162 | TTCAAGCAGAGGCGGTGTTGACTC |
| TDR | 32,822,178 | 32,822,201 | GGCCTTCCTCAAGATACAAGCATC |
PCR Primers
| Primer | Sequence |
| SU | GGTTGCGGAGTCTTCTGC |
| SD | TTGGCTCATAAGACCTTTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17710A-D7 | VGB6 | C57BL/6NTac | |
| 17710A-E5 | VGB6 | C57BL/6NTac | |
| 17710A-F8 | VGB6 | C57BL/6NTac | |
| 17710A-F10 | VGB6 | C57BL/6NTac | |
