| Gene | Xiap |
| Chromosome | X |
| Deletion Start | 42,105,080 |
| Deletion End | 42,094,402 |
| Deletion Size | 10,679 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 42,094,542 | 42,094,563 | GCGAGCTGGGTTTCTTTATACC |
| TUP | 42,094,566 | 42,094,591 | TGAAGGAGACACCGTGCAATGTTTCA |
| TUR | 42,094,613 | 42,094,635 | AGCTGAGTCTCCATACTGCCATC |
| TDF | 42,104,337 | 42,104,361 | AGCCCTCACATCGAAGCACTAAATC |
| TDP | 42,104,364 | 42,104,387 | ACCTGTCCCACAGTAGAAGCAGCC |
| TDR | 42,104,405 | 42,104,428 | GTTGAAAGGCATGGGTAATTCCAG |
PCR Primers
| Primer | Sequence |
| SU | TGAGAGAGAAACAGGCTGGG |
| SD | CCAATTAAAGTCATTGGGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17711A-B7 | VGB6 | C57BL/6NTac | |
| 17711A-C5 | VGB6 | C57BL/6NTac | |
| 17711A-G2 | VGB6 | C57BL/6NTac | |
