| Gene | Xylb |
| Chromosome | 9 |
| Deletion Start | 119,391,915 |
| Deletion End | 119,357,609 |
| Deletion Size | 34,307 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 119,358,389 | 119,358,406 | TCAGCCCGATGAGGAGAG |
| TUP | 119,358,407 | 119,358,426 | TTGGAACCTGCGGCTCCCAC |
| TUR | 119,358,467 | 119,358,487 | GGGAAGATGGAGAAACAGGTC |
| TDF | 119,391,678 | 119,391,697 | CAAGCAGCCGTAAGAGACAC |
| TDP | 119,391,706 | 119,391,733 | CCCTGTTACATACATTGCTCTTCCTGCC |
| TDR | 119,391,741 | 119,391,759 | GGAGAAGGGCCGCATAGAC |
PCR Primers
| Primer | Sequence |
| SU | GGTCGGCGCAGGGGTGAG |
| SD | GGTCACAGGCAGGAGAGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17712A-C5 | VGB6 | C57BL/6NTac | |
| 17712A-C9 | VGB6 | C57BL/6NTac | |
| 17712A-E11 | VGB6 | C57BL/6NTac | |
| 17712A-E1 | VGB6 | C57BL/6NTac | |
