| Gene | Zbtb7a |
| Chromosome | 10 |
| Deletion Start | 81,148,483 |
| Deletion End | 81,143,977 |
| Deletion Size | 4,507 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 81,144,160 | 81,144,178 | TTCACGTCGGGAGCTGTAG |
| TUP | 81,144,179 | 81,144,202 | TGGACCAGCAGAACGTGTACGAGA |
| TUR | 81,144,205 | 81,144,223 | CCTCGGCACTCACGAAGTC |
| TDF | 81,147,672 | 81,147,692 | TGGTGTCACCTGTCATAGTTC |
| TDP | 81,147,693 | 81,147,715 | TGGGTCTGACATCTGGCAGGCAC |
| TDR | 81,147,749 | 81,147,767 | AGCCAGGCAGAGGCTGTTG |
PCR Primers
| Primer | Sequence |
| SU | CTTGGTTTCCTCTGCTGCTC |
| SD | TAAGTGCCCTGAGGAAGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 64 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17714A-G4 | VGB6 | C57BL/6NTac | |
| 17714A-E10 | VGB6 | C57BL/6NTac | |
| 17714A-A10 | VGB6 | C57BL/6NTac | |
| 17714A-E7 | VGB6 | C57BL/6NTac | |
| 17714A-C6 | VGB6 | C57BL/6NTac | |
