| Gene | Zc3h3 |
| Chromosome | 15 |
| Deletion Start | 75,841,892 |
| Deletion End | 75,809,531 |
| Deletion Size | 32,362 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,841,369 | 75,841,390 | TGGCCTTCCTATTTGAGACTAC |
| TUP | 75,841,345 | 75,841,367 | TCGTGCGTTTCCCTAACACCCGA |
| TUR | 75,841,322 | 75,841,339 | CGAATCCTGCCAGACACC |
| TDF | 75,809,938 | 75,809,955 | TTGCCCAGGGCTCACTTG |
| TDP | 75,809,913 | 75,809,935 | AGGCTTCTGCTCTCACTGGGAGC |
| TDR | 75,809,862 | 75,809,884 | GCCTAGAACATCCTCACAACCAC |
PCR Primers
| Primer | Sequence |
| SU | CCAAGTCTTCCGAGGAACC |
| SD | GTGGTCCCACCAATTAGTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17715A-B4 | VGB6 | C57BL/6NTac | |
| 17715A-F6 | VGB6 | C57BL/6NTac | |
