| Gene | Zfp36l2 |
| Chromosome | 17 |
| Deletion Start | 84,187,691 |
| Deletion End | 84,185,658 |
| Deletion Size | 2,034 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,187,665 | 84,187,687 | CCACACTTCTGTCACCCTTCTAC |
| TUP | 84,187,634 | 84,187,661 | ATCGACTTCTTGTGCAAGGTAAGCAGGC |
| TUR | 84,187,581 | 84,187,602 | GGCAAGTCTCGGAACTCCAAAG |
| TDF | 84,186,611 | 84,186,633 | CCAAGTACAAGACAGAGCTGTGC |
| TDP | 84,186,589 | 84,186,610 | CGCACCTTCCACACCATCGGCT |
| TDR | 84,186,545 | 84,186,563 | GTCCGCGTTGTGGATGAAG |
PCR Primers
| Primer | Sequence |
| SU | CTCAGGTCTCCGAGTTCCAC |
| SD | TAGTTTAGGGAGGTGGCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 107 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17716A-B8 | VGB6 | C57BL/6NTac | |
| 17716A-F2 | VGB6 | C57BL/6NTac | |
| 17716A-D7 | VGB6 | C57BL/6NTac | |
| 17716A-C2 | VGB6 | C57BL/6NTac | |
| 17716A-D2 | VGB6 | C57BL/6NTac | |
| 17716A-A11 | VGB6 | C57BL/6NTac | |
