17717   Zfp384     Status: ES cell colonies screened / QC positives

GeneZfp384
Chromosome6
Deletion Start125,036,815
Deletion End125,023,687
Deletion Size13,129
Genome Build38
MGI IDMGI:2443203
NCBI Gene ID269800
BAC ID295I1
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 6

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF125,023,710125,023,731CCCGTACTTCTGGCCTTCTATC
TUP125,023,732125,023,753CCCACAGTCTCAGGGCAGGTGA
TUR125,023,795125,023,816CAGCGTGACGGACTTACTACTC
TDF125,036,288125,036,310CATACCCATCTCCCTCAGGAAAC
TDP125,036,317125,036,346TATGAAACATATGCGCAAACACAACCCTCC
TDR125,036,354125,036,373GCTGCTTGCACTTGCTGTTG

PCR Primers

PrimerSequence
SUCTGTTCTCAGGCTGTGGATG
SDTGGTGGCATTTAGAGCTTCC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR107 bp++-
TDFTDR86 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
17717A-H6VGB6C57BL/6NTac
17717A-F3VGB6C57BL/6NTac
17717A-B5VGB6C57BL/6NTac
17717A-B7VGB6C57BL/6NTac
17717A-F1VGB6C57BL/6NTac
17717A-E2VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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