| Gene | Zfp810 |
| Chromosome | 9 |
| Deletion Start | 22,290,538 |
| Deletion End | 22,280,459 |
| Deletion Size | 10,080 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 22,289,979 | 22,290,002 | CAATCCTTAAACCTAGCCTTGGAC |
| TUP | 22,289,959 | 22,289,978 | TTACAGCCTGTGGGCCAGGC |
| TUR | 22,289,933 | 22,289,954 | CCCTAAGACAGGCACTCATCAG |
| TDF | 22,280,778 | 22,280,802 | CGAGTGTTTCCTTACCATGTGTTTC |
| TDP | 22,280,750 | 22,280,774 | AGCACTGCAATGCAAGTGAGAACCA |
| TDR | 22,280,707 | 22,280,728 | TGGAATTACACTGCACACTGAC |
PCR Primers
| Primer | Sequence |
| SU | CTGAGCCAGCCTTTAACTGG |
| SD | CCTCAACGAGAAGCTCATTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17718A-C11 | VGB6 | C57BL/6NTac | |
| 17718A-D11 | VGB6 | C57BL/6NTac | |
| 17718A-A4 | VGB6 | C57BL/6NTac | |
| 17718A-D2 | VGB6 | C57BL/6NTac | |
| 17718A-D7 | VGB6 | C57BL/6NTac | |
| 17718A-F7 | VGB6 | C57BL/6NTac | |
