| Gene | Zkscan17 |
| Chromosome | 11 |
| Deletion Start | 59,503,770 |
| Deletion End | 59,487,012 |
| Deletion Size | 16,759 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 59,503,112 | 59,503,130 | GCCCTGATCTGCTGCTTTC |
| TUP | 59,503,088 | 59,503,111 | TGGGATTGCAGCTCAAGCACTGTG |
| TUR | 59,503,061 | 59,503,079 | CGCCATCGTCAATCACCAC |
| TDF | 59,487,613 | 59,487,634 | AGGTGCAAACCTCACAGAAATC |
| TDP | 59,487,585 | 59,487,610 | ACGTGTGCCCAAACTGTGGAAAGATC |
| TDR | 59,487,552 | 59,487,572 | CAGGTGCCGGATGAAGTTGAC |
PCR Primers
| Primer | Sequence |
| SU | TGCCAGTGTCCTGTTGAATC |
| SD | AAATGTGACCTCCTGATGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17719A-D6 | VGB6 | C57BL/6NTac | |
| 17719A-F7 | VGB6 | C57BL/6NTac | |
| 17719A-F8 | VGB6 | C57BL/6NTac | |
| 17719A-F11 | VGB6 | C57BL/6NTac | |
| 17719A-G12 | VGB6 | C57BL/6NTac | |
| 17719A-H10 | VGB6 | C57BL/6NTac | |
