| Gene | 1700037H04Rik |
| Chromosome | 2 |
| Deletion Start | 131,153,609 |
| Deletion End | 131,146,789 |
| Deletion Size | 6,821 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 131,153,354 | 131,153,376 | ATATGCCAGTCCCTCTGTTCCAC |
| TUP | 131,153,325 | 131,153,350 | TCGCAGTGTTAAAGGACATGAGGGCT |
| TUR | 131,153,296 | 131,153,315 | GGCCCAGGTGTCTGATGTTC |
| TDF | 131,147,676 | 131,147,695 | GCGCCAGTTTCAAGGGTATG |
| TDP | 131,147,649 | 131,147,674 | AGCCGAGCACTGGCTTCAATTCCACA |
| TDR | 131,147,608 | 131,147,627 | CGTCCGCTAAACAGCAAGTG |
PCR Primers
| Primer | Sequence |
| SU | CAGTAGGGTATCAGGCAGGG |
| SD | ACAACCAGCAGTGGAGAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17720A-H5 | VGB6 | C57BL/6NTac | |
| 17720A-B5 | VGB6 | C57BL/6NTac | |
| 17720A-H2 | VGB6 | C57BL/6NTac | |
| 17720A-C4 | VGB6 | C57BL/6NTac | |
| 17720A-C9 | VGB6 | C57BL/6NTac | |
