| Gene | Pinx1 |
| Chromosome | 14 |
| Deletion Start | 63,872,390 |
| Deletion End | 63,860,432 |
| Deletion Size | 11,959 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 63,860,643 | 63,860,665 | CAGACTGTTGTTCCTGGAGATTC |
| TUP | 63,860,673 | 63,860,696 | TTTCCAGGCTCGAACCAAGTGTCC |
| TUR | 63,860,720 | 63,860,739 | GGGCCTGAAGCGATGCTATG |
| TDF | 63,871,644 | 63,871,663 | GGAGAGCCATGTCGGTTGTG |
| TDP | 63,871,665 | 63,871,690 | CCCTCTCCTTGGAGGAATGCCTGTGG |
| TDR | 63,871,720 | 63,871,739 | TGTGACCTGAGCCTGGATTG |
PCR Primers
| Primer | Sequence |
| SU | CAGAGTTAAACTGCGAACTGC |
| SD | GTTCTTGGAACCACTGCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17721A-B1 | VGB6 | C57BL/6NTac | |
| 17721A-D11 | VGB6 | C57BL/6NTac | |
