| Gene | Acox1 |
| Chromosome | 11 |
| Deletion Start | 116,198,701 |
| Deletion End | 116,173,453 |
| Deletion Size | 25,249 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 116,198,639 | 116,198,660 | TCCAGAGTTAATCACGCACATC |
| TUP | 116,198,614 | 116,198,638 | TTGGATGGTAGTCCGGAGAACACCC |
| TUR | 116,198,585 | 116,198,604 | TCCAGGCTCACCGATTTCTC |
| TDF | 116,174,367 | 116,174,384 | TGACCCTTGGCTCTGTTC |
| TDP | 116,174,344 | 116,174,366 | TCGGCCGCTATGATGGCAATGTG |
| TDR | 116,174,310 | 116,174,328 | GGGACTTCTTGGCCCACTC |
PCR Primers
| Primer | Sequence |
| SU | TTTACCTTGCTTTCCTTGGC |
| SD | TGTAAGGGCCACACACTCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17722A-C5 | VGB6 | C57BL/6NTac | |
| 17722A-H4 | VGB6 | C57BL/6NTac | |
