Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

17723 Actr6 Status: ES cell colonies screened / QC positives

Gene	Actr6
Chromosome	10
Deletion Start	89,732,238
Deletion End	89,712,429
Deletion Size	19,810
Genome Build	38

MGI ID	MGI:1914269
NCBI Gene ID	67019
BAC ID	125A24
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 10

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	89,731,517	89,731,535	AAGATCCTCCTGCCTCCAC
TUP	89,731,488	89,731,516	CTCCTGGTTGCTAGGATTACAGCTGAGGG
TUR	89,731,457	89,731,478	GTCAAATGCCCACTGAGTTCTG
TDF	89,713,100	89,713,124	TGTTCTCTTCACCTGTTCTGATCTC
TDP	89,713,068	89,713,091	AAGCGGTCGAGCTGAGTCAGCACA
TDR	89,713,026	89,713,049	CATGCAGTTAGTTTGGCTTTGCTG

PCR Primers

Primer	Sequence
SU	CTCAATTCTTCAAGGCTCGG
SD	AAGAATCTATTCTCAGGAACAG
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	bp	-	+	+
TUF	TUR	79 bp	+	+	-
TDF	TDR	99 bp	+	+	-
NeoFwd	SD	bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
17723A-A9	VGB6	C57BL/6NTac
17723A-B2	VGB6	C57BL/6NTac
17723A-D2	VGB6	C57BL/6NTac
17723A-G7	VGB6	C57BL/6NTac
17723A-E6	VGB6	C57BL/6NTac