| Gene | Agxt2l2 |
| Chromosome | 11 |
| Deletion Start | 51,586,715 |
| Deletion End | 51,584,824 |
| Deletion Size | 1,892 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 51,585,615 | 51,585,635 | TCAACAACGTGGCTCATGGTC |
| TUP | 51,585,638 | 51,585,661 | TATGACGCCTCTCACCCTTGGTGA |
| TUR | 51,585,694 | 51,585,715 | TCTGTTCAACCCAGGCTATCAG |
| TDF | 51,586,586 | 51,586,604 | CCAAGCCGCACATGAACAG |
| TDP | 51,586,606 | 51,586,632 | ACCTAGTGCTCAACACCAACAGCCGCT |
| TDR | 51,586,655 | 51,586,673 | CGACAGCCTCTGGGCATAG |
PCR Primers
| Primer | Sequence |
| SU | GAAGTCCGACCATGGATCAG |
| SD | CTTCACTAAGTCCCAATGTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17724A-A9 | VGB6 | C57BL/6NTac | |
| 17724A-B5 | VGB6 | C57BL/6NTac | |
| 17724A-G2 | VGB6 | C57BL/6NTac | |
| 17724A-G8 | VGB6 | C57BL/6NTac | |
| 17724A-F8 | VGB6 | C57BL/6NTac | |
| 17724A-F10 | VGB6 | C57BL/6NTac | |
