| Gene | Ahsa2 |
| Chromosome | 11 |
| Deletion Start | 23,497,851 |
| Deletion End | 23,490,319 |
| Deletion Size | 7,533 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,497,391 | 23,497,408 | CTCTTTGGCGGGCATTGG |
| TUP | 23,497,366 | 23,497,388 | AAAGCTGAGGGAAGCAGAGCCCA |
| TUR | 23,497,330 | 23,497,352 | TCTTTCAGGACAGCTAAGTCATC |
| TDF | 23,490,479 | 23,490,503 | CAAGGGAGTTCCTGTCTGTAAAGAG |
| TDP | 23,490,441 | 23,490,469 | AAATTCTGTTGGCAGAAGCAGCACTTCGA |
| TDR | 23,490,398 | 23,490,418 | TCAAGCATTCTGGGCGGTAAG |
PCR Primers
| Primer | Sequence |
| SU | CTAGCAGAAGACAGCGCCAG |
| SD | TGTAACAGAATGCAGCCACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17725A-C6 | VGB6 | C57BL/6NTac | |
| 17725A-D2 | VGB6 | C57BL/6NTac | |
| 17725A-G2 | VGB6 | C57BL/6NTac | |
| 17725A-G10 | VGB6 | C57BL/6NTac | |
| 17725A-H3 | VGB6 | C57BL/6NTac | |
| 17725A-H5 | VGB6 | C57BL/6NTac | |
