17727   Arhgap23     Status: ES cell colonies screened / QC positives

GeneArhgap23
Chromosome11
Deletion Start97,476,168
Deletion End97,451,530
Deletion Size24,639
Genome Build38
MGI IDMGI:3697726
NCBI Gene ID58996
BAC ID252L13
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF97,452,13497,452,155TGGATGACATCGGGTACATTGG
TUP97,452,16597,452,190CTATAGCCCATCATTCCAGCGCCGGA
TUR97,452,20897,452,227GGTGAGTCCCGGAAGGAGAG
TDF97,475,29897,475,317CCCGTTTCCTTCCTCCTGTC
TDP97,475,32497,475,352ATCCGGGATCTCCCAGGACACTACTATGA
TDR97,475,38397,475,401TGAGTGGTCAGCAATGGTC

PCR Primers

PrimerSequence
SUTTTGTGGCTAAAGGCTGTCC
SDCTTCCAATCTTGTTCCTCGTG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR94 bp++-
TDFTDR104 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
17727A-A7VGB6C57BL/6NTac
17727A-B5VGB6C57BL/6NTac
17727A-H11VGB6C57BL/6NTac
17727A-D2VGB6C57BL/6NTac
17727A-F8VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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