| Gene | Bag1 |
| Chromosome | 4 |
| Deletion Start | 40,947,791 |
| Deletion End | 40,936,524 |
| Deletion Size | 11,268 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 40,947,749 | 40,947,770 | CAGACGGAGGAAATGGAAACAC |
| TUP | 40,947,724 | 40,947,747 | CAGACTCAGCGTGATCGTCACCCA |
| TUR | 40,947,678 | 40,947,700 | CCACATATCCCAACCCGATCTAC |
| TDF | 40,936,660 | 40,936,680 | GCAGGTGTTCTTAGCAGAGTG |
| TDP | 40,936,633 | 40,936,659 | TGACACAGTGGAGCAATACATCTGCCA |
| TDR | 40,936,594 | 40,936,612 | GCCAGGGCCAAGTTTGTAG |
PCR Primers
| Primer | Sequence |
| SU | TTCTCAGAGCGAGAAGGTAG |
| SD | CCAGCTGCCAAACTTTATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17729A-H8 | VGB6 | C57BL/6NTac | |
| 17729A-G6 | VGB6 | C57BL/6NTac | |
| 17729A-G8 | VGB6 | C57BL/6NTac | |
| 17729A-C4 | VGB6 | C57BL/6NTac | |
| 17729A-F2 | VGB6 | C57BL/6NTac | |
