| Gene | Brpf3 |
| Chromosome | 17 |
| Deletion Start | 28,836,781 |
| Deletion End | 28,805,958 |
| Deletion Size | 30,824 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,806,643 | 28,806,666 | TGTTCTGCGACATCTGCAACTTAG |
| TUP | 28,806,667 | 28,806,689 | CTGTGCACCAGGAGTGCTACGGC |
| TUR | 28,806,717 | 28,806,735 | GCAGGCAGCAGCGACATAG |
| TDF | 28,836,487 | 28,836,506 | CCTCAGCTCTGCTTGCATTC |
| TDP | 28,836,507 | 28,836,529 | TAGGCAGTGGCTTCCAAGGGACA |
| TDR | 28,836,570 | 28,836,589 | TGCGGCCTTCTAGCATCTTG |
PCR Primers
| Primer | Sequence |
| SU | GTGCCGTGTGAATGCTTTAG |
| SD | TGCCCTGAGGTATGGTTAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17730A-B5 | VGB6 | C57BL/6NTac | |
| 17730A-H11 | VGB6 | C57BL/6NTac | |
| 17730A-H10 | VGB6 | C57BL/6NTac | |
| 17730A-F5 | VGB6 | C57BL/6NTac | |
| 17730A-H7 | VGB6 | C57BL/6NTac | |
| 17730A-F7 | VGB6 | C57BL/6NTac | |
