| Gene | Cep135 |
| Chromosome | 5 |
| Deletion Start | 76,621,356 |
| Deletion End | 76,591,762 |
| Deletion Size | 29,595 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,592,331 | 76,592,355 | GAGTCATGTAATGCTCTGTGAAGTC |
| TUP | 76,592,358 | 76,592,382 | TGTACTCTCAGCCTTGCTGTCTTGC |
| TUR | 76,592,389 | 76,592,408 | CACGCTACAGGCAGAAGGAG |
| TDF | | | GTGTGTGTGTGTGTGTAAGCAG |
| TDP | 76,620,975 | 76,621,003 | ACATCTCCTCTTGTGAGAGTGCATTGTGA |
| TDR | 76,621,020 | 76,621,043 | AACAGGCACCATAGAGAGAACAAC |
PCR Primers
| Primer | Sequence |
| SU | AGTGGAATTGCAAGGGTCC |
| SD | AGAAACACTCCTCCTATGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17731A-A1 | VGB6 | C57BL/6NTac | |
| 17731A-D7 | VGB6 | C57BL/6NTac | |
| 17731A-G8 | VGB6 | C57BL/6NTac | |
| 17731A-H11 | VGB6 | C57BL/6NTac | |
| 17731A-H9 | VGB6 | C57BL/6NTac | |
