| Gene | Crkl |
| Chromosome | 16 |
| Deletion Start | 17,483,838 |
| Deletion End | 17,452,481 |
| Deletion Size | 31,358 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 17,452,740 | 17,452,761 | ACAAGATCCACTACCTGGACAC |
| TUP | 17,452,766 | 17,452,789 | ACCTTAATCGAACCGGCGCCCAGG |
| TUR | 17,452,819 | 17,452,838 | CTTCGCATCCGGTCAAGTTC |
| TDF | 17,483,227 | 17,483,247 | AGCACCACACCTGGCTACTTC |
| TDP | 17,483,249 | 17,483,273 | CACTTTCCTCCCAAGACAGGGTTGC |
| TDR | 17,483,299 | 17,483,319 | CCTCATGGACAGAGCGAGTTC |
PCR Primers
| Primer | Sequence |
| SU | GGACAGAAGTCACTGGAGCC |
| SD | AGCCAGACTTGAGGAGTTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17732B-A1 | VGB6 | C57BL/6NTac | |
| 17732B-A4 | VGB6 | C57BL/6NTac | |
| 17732B-B5 | VGB6 | C57BL/6NTac | |
| 17732B-H6 | VGB6 | C57BL/6NTac | |
