| Gene | Fam126b |
| Chromosome | 1 |
| Deletion Start | 58,558,024 |
| Deletion End | 58,529,611 |
| Deletion Size | 28,414 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 58,557,645 | 58,557,669 | TGCATGAATACTTAGGTGTGTTGTG |
| TUP | 58,557,613 | 58,557,642 | AGCATCAGAGAGGAACTGGATACATCCTGC |
| TUR | 58,557,591 | 58,557,611 | TTAAAGCTACCCGCCTGAGAG |
| TDF | 58,529,884 | 58,529,906 | GCCAGTGCTTCCTCAAGTAAATC |
| TDP | 58,529,849 | 58,529,878 | ACTATGTAAATGGCGGTCAAGCAAGTACCA |
| TDR | 58,529,823 | 58,529,841 | AACTCCCTCAGTGCCAACC |
PCR Primers
| Primer | Sequence |
| SU | TCAGCTTGCTACCTATATGACCC |
| SD | AATCTCTGAGGCACTCCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17735A-F10 | VGB6 | C57BL/6NTac | |
| 17735A-D7 | VGB6 | C57BL/6NTac | |
| 17735A-B6 | VGB6 | C57BL/6NTac | |
| 17735A-G10 | VGB6 | C57BL/6NTac | |
