| Gene | Flad1 |
| Chromosome | 3 |
| Deletion Start | 89,411,241 |
| Deletion End | 89,403,083 |
| Deletion Size | 8,159 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 89,411,070 | 89,411,091 | GAGGGAAACCGGAGTAGGAATC |
| TUP | 89,411,045 | 89,411,068 | CCTTTCTTGCCAAAGTCCGGCCCT |
| TUR | 89,410,997 | 89,411,015 | CACTCAACCGAAGGGTCAC |
| TDF | 89,403,449 | 89,403,472 | CAGGACTGGACCTACAGAAACATC |
| TDP | 89,403,420 | 89,403,442 | TTTCTGCGGCAGCTGTTTGTCCC |
| TDR | 89,403,376 | 89,403,395 | CAGCCCGTCAACCACCTTAC |
PCR Primers
| Primer | Sequence |
| SU | TTAAAGGGACAGGTGATCCG |
| SD | AAGAAGGTTCTGGGCCTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17737A-B2 | VGB6 | C57BL/6NTac | |
| 17737A-C7 | VGB6 | C57BL/6NTac | |
| 17737A-G10 | VGB6 | C57BL/6NTac | |
| 17737A-E6 | VGB6 | C57BL/6NTac | |
| 17737A-F5 | VGB6 | C57BL/6NTac | |
| 17737A-D8 | VGB6 | C57BL/6NTac | |
