| Gene | Gdap2 |
| Chromosome | 3 |
| Deletion Start | 100,170,003 |
| Deletion End | 100,205,835 |
| Deletion Size | 35,833 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,170,887 | 100,170,908 | TGGTCTGTCAGGAAAGGAGATG |
| TUP | 100,170,909 | 100,170,932 | TGGCATTACTGAACTGCACAGCCA |
| TUR | 100,170,935 | 100,170,958 | GAGGCTTTCATTGCTGGTATTCAC |
| TDF | 100,205,587 | 100,205,606 | TTGCGGCTTCTACCTTACAC |
| TDP | 100,205,611 | 100,205,635 | TCTGACACGCTCCATTGGAACAGGT |
| TDR | 100,205,643 | 100,205,661 | CATGCAGCGTGGAGCAGAC |
PCR Primers
| Primer | Sequence |
| SU | GGCTGAAAGGGTTGAGTGAG |
| SD | CTATGAACAATGGTGGCGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17740A-E4 | VGB6 | C57BL/6NTac | |
| 17740A-E8 | VGB6 | C57BL/6NTac | |
