| Gene | Gm5134 |
| Chromosome | 10 |
| Deletion Start | 75,995,927 |
| Deletion End | 75,976,315 |
| Deletion Size | 19,613 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,976,864 | 75,976,886 | AGCCTCAGAGCCCACAGTACATC |
| TUP | 75,976,887 | 75,976,911 | AATAAGGCCTCCGTCAGCAGCACCG |
| TUR | 75,976,925 | 75,976,947 | ACTCTTCAGCATCCCTGTCATTC |
| TDF | 75,994,955 | 75,994,974 | TCTGGAGTGTTGGGCGAATG |
| TDP | 75,994,976 | 75,994,999 | TTCCTCTGGATGCTGGGTGAGCTC |
| TDR | 75,995,002 | 75,995,022 | GGCTGTGGAGCAGGTAAGTAG |
PCR Primers
| Primer | Sequence |
| SU | TTGATGTCTGAGGCCCTAGC |
| SD | AACAGTTTTCCTCAGGTTTAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17743A-A2 | VGB6 | C57BL/6NTac | |
| 17743A-A4 | VGB6 | C57BL/6NTac | |
| 17743A-A5 | VGB6 | C57BL/6NTac | |
| 17743A-E8 | VGB6 | C57BL/6NTac | |
| 17743A-F7 | VGB6 | C57BL/6NTac | |
