| Gene | Gpn3 |
| Chromosome | 5 |
| Deletion Start | 122,382,330 |
| Deletion End | 122,372,612 |
| Deletion Size | 9,719 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 122,372,862 | 122,372,880 | GTCCAGCACCCAGACAGAG |
| TUP | 122,372,881 | 122,372,904 | CTGCAGGACACCTTTGCTCCAGGA |
| TUR | 122,372,914 | 122,372,937 | GGATATGAAGACACCTGGAACGAC |
| TDF | 122,381,993 | 122,382,011 | CGGTGGGCATGGTCTGAAG |
| TDP | 122,382,014 | 122,382,037 | CTGTCAGGGCCGCTTCTCTAACCC |
| TDR | 122,382,071 | 122,382,091 | GACTGGTCGTATGGCAGGAAG |
PCR Primers
| Primer | Sequence |
| SU | CAACCGATAACGGCTCTCG |
| SD | TACGTGTGTGGCTAGGTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17745A-D1 | VGB6 | C57BL/6NTac | |
| 17745A-F10 | VGB6 | C57BL/6NTac | |
| 17745A-H6 | VGB6 | C57BL/6NTac | |
