| Gene | Mdh1 |
| Chromosome | 11 |
| Deletion Start | 21,571,729 |
| Deletion End | 21,557,431 |
| Deletion Size | 14,299 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 21,571,250 | 21,571,273 | GATCTCCGTTTGTAATGACAGGAC |
| TUP | 21,571,224 | 21,571,248 | AGCATCGCTAGTCAAACAGTGCCGA |
| TUR | 21,571,164 | 21,571,187 | GATGGTTACGTTACATCTGCAAGG |
| TDF | 21,557,649 | 21,557,670 | GGTGTGCTTTGAGTTACCATTC |
| TDP | 21,557,623 | 21,557,647 | AAGCTAGGCTATCCCTGCTGGTCCC |
| TDR | 21,557,578 | 21,557,597 | GACAAGGGCGAGAGCTTCAG |
PCR Primers
| Primer | Sequence |
| SU | TCGAGTTAACGCCTCCTCTC |
| SD | CTGAAATTGATTGGGCTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17756A-C1 | VGB6 | C57BL/6NTac | |
| 17756A-H9 | VGB6 | C57BL/6NTac | |
| 17756A-D8 | VGB6 | C57BL/6NTac | |
| 17756A-C8 | VGB6 | C57BL/6NTac | |
