| Gene | Mettl9 |
| Chromosome | 7 |
| Deletion Start | 121,057,397 |
| Deletion End | 121,034,814 |
| Deletion Size | 22,584 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 121,035,528 | 121,035,547 | TCTGCCTATGAGACGTGTCC |
| TUP | 121,035,550 | 121,035,577 | TCAACTTGGGTTTAGTGAGCTGACCAGA |
| TUR | 121,035,585 | 121,035,607 | TTGCATCATCTTCCTCCTCATCC |
| TDF | 121,056,480 | 121,056,500 | TAAATGCAGTGCCCAGTACAC |
| TDP | 121,056,516 | 121,056,544 | TACAGTGGCAAGAACTTGTAATCCTGGCA |
| TDR | 121,056,545 | 121,056,568 | GGAGTCTCCTGACTGTCTCCATAG |
PCR Primers
| Primer | Sequence |
| SU | CTGCGTTTGTACGTGCAC |
| SD | GATACAGAGGCACTGATGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17757A-C1 | VGB6 | C57BL/6NTac | |
| 17757A-C4 | VGB6 | C57BL/6NTac | |
| 17757A-E10 | VGB6 | C57BL/6NTac | |
| 17757A-G4 | VGB6 | C57BL/6NTac | |
| 17757A-E11 | VGB6 | C57BL/6NTac | |
