| Gene | Mrto4 |
| Chromosome | 4 |
| Deletion Start | 139,352,293 |
| Deletion End | 139,347,654 |
| Deletion Size | 4,640 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 139,351,437 | 139,351,459 | AGTGAACAAAGTGGGCTTAGAGG |
| TUP | 139,351,405 | 139,351,429 | CCAGCCTTTGGTGTGGCTTTGCTTT |
| TUR | 139,351,361 | 139,351,382 | GCCTTGCTGCCTTCATATTCTC |
| TDF | 139,348,290 | 139,348,312 | CTGGAGAGGTGGCTTAGAGTTGG |
| TDP | 139,348,259 | 139,348,286 | TACTGACTGCTCTTCCAGAGGACATGGG |
| TDR | 139,348,221 | 139,348,240 | GTGCCTGAGAGGGCCTGATG |
PCR Primers
| Primer | Sequence |
| SU | AGATCCAGAACCCAGACGC |
| SD | CAACTGGGTTGGACCAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17759A-E8 | VGB6 | C57BL/6NTac | |
| 17759A-B5 | VGB6 | C57BL/6NTac | |
| 17759A-B11 | VGB6 | C57BL/6NTac | |
| 17759A-C5 | VGB6 | C57BL/6NTac | |
| 17759A-E4 | VGB6 | C57BL/6NTac | |
| 17759A-A10 | VGB6 | C57BL/6NTac | |
