| Gene | Nap1l1 |
| Chromosome | 10 |
| Deletion Start | 111,496,098 |
| Deletion End | 111,481,303 |
| Deletion Size | 14,796 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 111,482,170 | 111,482,189 | AGCAGTGCTGGGCTTGAGTG |
| TUP | 111,482,198 | 111,482,227 | TTGAGGCTCTACAAGGGTTTGTTCTGATGG |
| TUR | 111,482,231 | 111,482,251 | GTTTGCTTACACCCGTGTTCC |
| TDF | 111,495,355 | 111,495,378 | GATCCAGACTATGACCCAAAGGTG |
| TDP | 111,495,379 | 111,495,400 | AGCAGAACTGTGCTGGCCTCCC |
| TDR | 111,495,415 | 111,495,438 | GATCTGTCTCATCTACGCCTACTC |
PCR Primers
| Primer | Sequence |
| SU | CTCTTAGATTTGGGGTTTTATTG |
| SD | TAATGCAGGCGGACTTCAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17760A-B12 | VGB6 | C57BL/6NTac | |
| 17760A-G2 | VGB6 | C57BL/6NTac | |
| 17760A-D8 | VGB6 | C57BL/6NTac | |
| 17760A-B6 | VGB6 | C57BL/6NTac | |
