| Gene | Prkrip1 |
| Chromosome | 5 |
| Deletion Start | 136,198,897 |
| Deletion End | 136,181,323 |
| Deletion Size | 17,575 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 136,198,464 | 136,198,484 | CCCGGCTCCTCTTACCTTATC |
| TUP | 136,198,440 | 136,198,463 | TTCCCTACGAGCTGGGATGACAGC |
| TUR | 136,198,403 | 136,198,425 | GAAAGGGCTGTGAAGCAATACTG |
| TDF | 136,182,105 | 136,182,129 | ACCATAAGGTATCAGGAGAGGAGTC |
| TDP | 136,182,076 | 136,182,100 | CGCCATTTGAGGACAACCAGGAAGC |
| TDR | 136,182,050 | 136,182,072 | GGGCTGTACCAACAATGCCAATG |
PCR Primers
| Primer | Sequence |
| SU | TCTTCCTCTCCGGATGTGAG |
| SD | AACAGGACCTGCCACATTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17763A-G5 | VGB6 | C57BL/6NTac | |
| 17763A-A4 | VGB6 | C57BL/6NTac | |
| 17763A-B1 | VGB6 | C57BL/6NTac | |
| 17763A-F2 | VGB6 | C57BL/6NTac | |
| 17763A-B8 | VGB6 | C57BL/6NTac | |
| 17763A-A2 | VGB6 | C57BL/6NTac | |
