| Gene | Prmt2 |
| Chromosome | 10 |
| Deletion Start | 76,236,762 |
| Deletion End | 76,207,689 |
| Deletion Size | 29,074 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,236,724 | 76,236,741 | TGCAGCGAGTCACAGGTC |
| TUP | 76,236,698 | 76,236,722 | TCCCGGTTCTGGTACGTAAGAGCCA |
| TUR | 76,236,663 | 76,236,686 | AAGCTGGTACTGTTCCTCTGTATG |
| TDF | 76,208,143 | 76,208,163 | GAGAAGTCCTACGCCTGTCTG |
| TDP | 76,208,117 | 76,208,139 | TTGCTACCTGCTCACACGGGAGA |
| TDR | 76,208,085 | 76,208,109 | ATGGAATATGTCCTTCACACCTCAC |
PCR Primers
| Primer | Sequence |
| SU | TTCTGCCTGTGGGTTAGGTC |
| SD | AGACATTACACGCGGCTACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17764A-A3 | VGB6 | C57BL/6NTac | |
| 17764A-B1 | VGB6 | C57BL/6NTac | |
| 17764A-D1 | VGB6 | C57BL/6NTac | |
| 17764A-F5 | VGB6 | C57BL/6NTac | |
