| Gene | Rbm17 |
| Chromosome | 2 |
| Deletion Start | 11,600,738 |
| Deletion End | 11,585,579 |
| Deletion Size | 15,160 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 11,600,397 | 11,600,418 | ATGCCTGAGACTGACTGCAAAC |
| TUP | 11,600,371 | 11,600,391 | ACAGATGCTGTTTTCATACCC |
| TUR | 11,600,288 | 11,600,309 | TCTGCAAAGCTCAATTCCAAAC |
| TDF | 11,585,746 | 11,585,769 | GATCCCTGTATTAACTGACCGTTG |
| TDP | 11,585,719 | 11,585,743 | TTGTTTGCCTTTCAGCTGTGGTGGA |
| TDR | 11,585,632 | 11,585,652 | TGCTCTGCTAGATCCAAGACC |
PCR Primers
| Primer | Sequence |
| SU | CTGAGGACTGTTTTTAAACTAG |
| SD | AAAGCAAGCGTCTTCACCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 131 bp | + | + | - |
| TDF | TDR | 138 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17767A-A12 | VGB6 | C57BL/6NTac | |
| 17767A-B1 | VGB6 | C57BL/6NTac | |
| 17767A-C4 | VGB6 | C57BL/6NTac | |
| 17767A-C3 | VGB6 | C57BL/6NTac | |
