| Gene | Rmnd5b |
| Chromosome | 11 |
| Deletion Start | 51,629,611 |
| Deletion End | 51,624,015 |
| Deletion Size | 5,597 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 51,629,564 | 51,629,582 | GGACAAGGTCCTGCACAAG |
| TUP | 51,629,539 | 51,629,562 | TCCTAACCTACGGGCAGCATTGCG |
| TUR | 51,629,516 | 51,629,534 | CAGCAGCTCCTCCAGACTC |
| TDF | 51,624,154 | 51,624,174 | ACGCTCTTGCCTTTGCCTTTC |
| TDP | 51,624,126 | 51,624,151 | TGCAGGCTAAAGTGTCCCTACTGTCC |
| TDR | 51,624,097 | 51,624,118 | GTTTCCCATCTGCTGGATTCTG |
PCR Primers
| Primer | Sequence |
| SU | GACTTCCTCTGGCACCCAC |
| SD | TCATTGGTAAGGAGAAGGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17768A-F10 | VGB6 | C57BL/6NTac | |
| 17768A-G8 | VGB6 | C57BL/6NTac | |
| 17768A-G1 | VGB6 | C57BL/6NTac | |
| 17768A-B8 | VGB6 | C57BL/6NTac | |
| 17768A-E9 | VGB6 | C57BL/6NTac | |
| 17768A-F12 | VGB6 | C57BL/6NTac | |
