| Gene | Shc1 |
| Chromosome | 3 |
| Deletion Start | 89,428,454 |
| Deletion End | 89,422,184 |
| Deletion Size | 6,271 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 89,422,252 | 89,422,271 | GACCAGACACGGGAGCTTTG |
| TUP | 89,422,273 | 89,422,295 | CAATAAGCCCACACGAGGCTGGC |
| TUR | 89,422,328 | 89,422,349 | TCACCCGAACCAAGTAGGAAAC |
| TDF | 89,428,309 | 89,428,328 | CCTCCCAGGTTCGGACAAAG |
| TDP | 89,428,331 | 89,428,356 | TCACCGCTTTGAGAGTGTCAGTCACC |
| TDR | 89,428,380 | 89,428,399 | GCAGAGATGATGGGCAAGTG |
PCR Primers
| Primer | Sequence |
| SU | GCCGAAGTACAACCCACTTC |
| SD | AGTGGGATATGTTATGCGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17769A-H10 | VGB6 | C57BL/6NTac | |
| 17769A-D12 | VGB6 | C57BL/6NTac | |
| 17769A-F3 | VGB6 | C57BL/6NTac | |
| 17769A-F7 | VGB6 | C57BL/6NTac | |
| 17769A-D10 | VGB6 | C57BL/6NTac | |
