| Gene | Snf8 |
| Chromosome | 11 |
| Deletion Start | 96,047,362 |
| Deletion End | 96,035,017 |
| Deletion Size | 12,346 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 96,035,175 | 96,035,194 | AGCCCAGGGAGGAGTGATTG |
| TUP | 96,035,198 | 96,035,222 | TGTCAGGAGGCTGCTGGTTAAGTCA |
| TUR | 96,035,263 | 96,035,282 | GATCGCCAAGGCTCCCATTG |
| TDF | 96,046,875 | 96,046,895 | TTGCTCCCTGCAAAGTATCTG |
| TDP | 96,046,905 | 96,046,932 | CAGAACACTGATCTCCACTTGCTCTGGG |
| TDR | 96,046,937 | 96,046,956 | GAAGTCCCTGCCTCTGTCTC |
PCR Primers
| Primer | Sequence |
| SU | ACCTTGGTAGGATGCATGGG |
| SD | CACCGCTTGCTTTCCTCTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17771A-H2 | VGB6 | C57BL/6NTac | |
| 17771A-B9 | VGB6 | C57BL/6NTac | |
| 17771A-B10 | VGB6 | C57BL/6NTac | |
| 17771A-E8 | VGB6 | C57BL/6NTac | |
| 17771A-E12 | VGB6 | C57BL/6NTac | |
| 17771A-D6 | VGB6 | C57BL/6NTac | |
