| Gene | St3gal2 |
| Chromosome | 8 |
| Deletion Start | 110,970,359 |
| Deletion End | 110,957,715 |
| Deletion Size | 12,645 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 110,958,179 | 110,958,199 | ACTCCCAGCACCCTGACAAAG |
| TUP | 110,958,200 | 110,958,222 | CAGGAAGCCCACCTGAGCTGAGC |
| TUR | 110,958,230 | 110,958,248 | GCAGCGGTCTGTGATCCAG |
| TDF | 110,970,195 | 110,970,212 | GTGCATGACGCCGACTTC |
| TDP | 110,970,215 | 110,970,239 | AGCCCACATCATCGACATGCTGGCC |
| TDR | 110,970,258 | 110,970,277 | GCTCAGTTCCCTCGGTAGAC |
PCR Primers
| Primer | Sequence |
| SU | CACAAGGGACTAGAGGGCTG |
| SD | GCACATGTGAGAGAAGCACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17772A-D3 | VGB6 | C57BL/6NTac | |
| 17772A-E9 | VGB6 | C57BL/6NTac | |
| 17772A-E6 | VGB6 | C57BL/6NTac | |
| 17772A-E12 | VGB6 | C57BL/6NTac | |
| 17772A-F3 | VGB6 | C57BL/6NTac | |
