| Gene | Tcea1 |
| Chromosome | 1 |
| Deletion Start | 4,878,127 |
| Deletion End | 4,857,917 |
| Deletion Size | 20,211 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,858,443 | 4,858,461 | TGCTTCCACACTTGACCTC |
| TUP | 4,858,462 | 4,858,488 | TCCAGCCAGCTTCACTTGAATCTCTTC |
| TUR | 4,858,492 | 4,858,509 | GCAAACCTGCTGCGTCTG |
| TDF | 4,877,986 | 4,878,005 | TGAAACGCATTGATGGACAC |
| TDP | 4,878,013 | 4,878,040 | TTGTTGCCTTGCAGTCCACAAGAATTGG |
| TDR | 4,878,044 | 4,878,062 | GCGAAGGGCATTGACAGAC |
PCR Primers
| Primer | Sequence |
| SU | CTACTGCCTGATCCCTCTGG |
| SD | ACTTCAGAAAACATGCATAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17773A-F11 | VGB6 | C57BL/6NTac | |
| 17773B-D5 | VGB6 | C57BL/6NTac | |
| 17773B-E1 | VGB6 | C57BL/6NTac | |
| 17773B-F12 | VGB6 | C57BL/6NTac | |
