| Gene | Tmem38b |
| Chromosome | 4 |
| Deletion Start | 53,860,137 |
| Deletion End | 53,826,112 |
| Deletion Size | 34,026 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 53,826,420 | 53,826,441 | GGCTTTGCGGTTTCTCAGTCAG |
| TUP | 53,826,442 | 53,826,461 | CCACTGGAGGCACGGTCCGG |
| TUR | 53,826,462 | 53,826,485 | AACTACATGCCAAGCGACTGTTAC |
| TDF | 53,860,001 | 53,860,023 | TGAAGTCAAACCGTCTTCCAATG |
| TDP | 53,860,028 | 53,860,049 | TGCCTCAAGTGCATCGAAGCGG |
| TDR | 53,860,085 | 53,860,107 | GCGACCCTCAGTCTTCTTTCTTG |
PCR Primers
| Primer | Sequence |
| SU | TTTGTGAAATTCCGTGAGTCC |
| SD | TTATGAGAATCTCTTACACCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17774A-A9 | VGB6 | C57BL/6NTac | |
| 17774A-B4 | VGB6 | C57BL/6NTac | |
| 17774A-H10 | VGB6 | C57BL/6NTac | |
| 17774A-A6 | VGB6 | C57BL/6NTac | |
| 17774A-H6 | VGB6 | C57BL/6NTac | |
