| Gene | Tor1a |
| Chromosome | 2 |
| Deletion Start | 30,967,851 |
| Deletion End | 30,960,932 |
| Deletion Size | 6,920 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,967,773 | 30,967,790 | AGCCCATCAGCCTGAGTC |
| TUP | 30,967,749 | 30,967,768 | CCTGGCCGGCGTACTCACCA |
| TUR | 30,967,716 | 30,967,738 | AGAGGCAGTAGAGACGAGGGTAG |
| TDF | 30,961,064 | 30,961,086 | CCCGAGGCTATGAAGTAGATGAG |
| TDP | 30,961,029 | 30,961,058 | CATCAGCAAGGTAGCGGAAGAGATGACGTT |
| TDR | 30,960,981 | 30,961,002 | ACAGTCTTGCAGCCCTTGTCAG |
PCR Primers
| Primer | Sequence |
| SU | ATAGGGCAGCAGTCATCATC |
| SD | ATGTCCAGGCTTGGGTTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17775A-D3 | VGB6 | C57BL/6NTac | |
| 17775A-G4 | VGB6 | C57BL/6NTac | |
| 17775A-F11 | VGB6 | C57BL/6NTac | |
| 17775A-A4 | VGB6 | C57BL/6NTac | |
| 17775A-A7 | VGB6 | C57BL/6NTac | |
| 17775A-B7 | VGB6 | C57BL/6NTac | |
