| Gene | Tsc1 |
| Chromosome | 2 |
| Deletion Start | 28,687,202 |
| Deletion End | 28,658,688 |
| Deletion Size | 28,515 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,659,169 | 28,659,188 | GTTGGGTGAGCTGAGAGTTC |
| TUP | 28,659,197 | 28,659,225 | CATTTGAAGGCAGCTAGCAGAATACTGGC |
| TUR | 28,659,251 | 28,659,270 | CACTGTGGGTGTGGGCTATC |
| TDF | 28,686,396 | 28,686,419 | GGCTTGGTGGATAAAGGGTTACTG |
| TDP | 28,686,423 | 28,686,447 | TTGCAGAGGACCTCAGTTTGGTTCC |
| TDR | 28,686,468 | 28,686,486 | TGAGGAGGCCAGAAGACAG |
PCR Primers
| Primer | Sequence |
| SU | CATAGTCCCTCGCTCACTCC |
| SD | AGCCACCCAGTTGTCTGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17776A-F5 | VGB6 | C57BL/6NTac | |
| 17776A-B5 | VGB6 | C57BL/6NTac | |
| 17776A-E2 | VGB6 | C57BL/6NTac | |
| 17776A-E4 | VGB6 | C57BL/6NTac | |
