| Gene | Wdr91 |
| Chromosome | 6 |
| Deletion Start | 34,910,816 |
| Deletion End | 34,880,704 |
| Deletion Size | 30,113 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,909,916 | 34,909,934 | GCAGGGAGCCAGTAATCAC |
| TUP | 34,909,885 | 34,909,913 | TGTCTCTTGGAATTCAGAAACTGTGGAGC |
| TUR | 34,909,842 | 34,909,862 | AAGAGCCAATGAGCCAGAGAC |
| TDF | 34,881,586 | 34,881,609 | CAGGTGTCATAGTGTTGCTAGGAG |
| TDP | 34,881,555 | 34,881,579 | TGTTTCTGATTCCCTGTGCAGTGCC |
| TDR | 34,881,530 | 34,881,553 | ACGTGTGAATGACCAGACGCTAAC |
PCR Primers
| Primer | Sequence |
| SU | CAAGGTTGGTAGGTTGGGC |
| SD | CCAGGCGACTCACATAGTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17779A-E9 | VGB6 | C57BL/6NTac | |
| 17779A-C8 | VGB6 | C57BL/6NTac | |
| 17779A-E2 | VGB6 | C57BL/6NTac | |
| 17779A-D4 | VGB6 | C57BL/6NTac | |
