| Gene | Zfp715 |
| Chromosome | 7 |
| Deletion Start | 43,311,162 |
| Deletion End | 43,297,486 |
| Deletion Size | 13,677 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,311,038 | 43,311,056 | CAGAGGAGCAGCCTGAGTC |
| TUP | 43,311,009 | 43,311,037 | CTGTGAGGTAAGCAGGAGTGACTTCATGA |
| TUR | 43,310,987 | 43,311,006 | CCCTGAAGACCCGAAGAACC |
| TDF | 43,297,774 | 43,297,792 | TGCAGCAACTGGGAAAGTC |
| TDP | 43,297,745 | 43,297,772 | TGGCTTCTAATGCCCAACTCTGACAGTA |
| TDR | 43,297,707 | 43,297,730 | GAGCACAAAGGTCTCTCCTAGATG |
PCR Primers
| Primer | Sequence |
| SU | CATGTGAGTGCTGCAGTGTG |
| SD | ACATTCCTCATGCATGTTTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17780A-A10 | VGB6 | C57BL/6NTac | |
| 17780A-G8 | VGB6 | C57BL/6NTac | |
| 17780A-G9 | VGB6 | C57BL/6NTac | |
| 17780A-F9 | VGB6 | C57BL/6NTac | |
| 17780A-F2 | VGB6 | C57BL/6NTac | |
